Preheat oven to 350 degrees.
Heat oil in a large pan and sauté onions and mushrooms until soft. Season with salt and pepper. Set aside to cool.
While the vegetables cool mix together the meatloaf.
In a large bowl combine the bread and milk until it forms a paste. Add eggs to the paste and mix until combined.
Next add ground turkey, 1/2 cup pizza sauce, pepperoni (make sure to save 6- 8 slices for the top of the meatloaf),1 cup mozzarella cheese, Italian seasoning, garlic powder, onion powder, salt, Worcestershire sauce, and romano cheese. Mix all the ingredients until combined.
If needed, drain the mushrooms and onions. Add vegetables to the meatloaf and mix until combined.
Form meatloaf mixture into a loaf on a pan sprayed with cooking spray. Spread 1/2 cup of pizza sauce over the top.
Cook for 1 hour.
Remove meatloaf from oven. Set oven to broil.
Sprinkle cheese and 6-8 slices of pepperoni on top of the meatloaf. Broil for 2-3 minutes or until cheese is melted and pepperoni is crisp.
Cut into slices and serve hot.